The minibus driver told Reuters earlier he was there to pick guests up.
It was not clear where they were being taken.
In total, over 700 holidaymakers were in isolation there.
Half a dozen guests including a baby in a pram exited the H10 Costa Adeje Palace' south of Tenerife, and underwent temperature scans and in the back of the hotel before boarding a 15-seater minibus, the video shot by hotel guest Christopher Betts showed.
One of the people boarding said he was from Belgium.
Spain's total number of coronavirus cases rose to 26 on Friday, with the bulk of them linked to Italy, hit by Europe's worst outbreak.